Development of a Rapid Automated Influenza A, Influenza B, and Respiratory Syncytial Virus A/B Multiplex Real-Time RT-PCR Assay and Its Use during the 2009 H1N1 Swine-Origin Influenza Virus Epidemic in Milwaukee, Wisconsin
06 Dec 2012 | Publications | Contributor(s): Beck, Jurgens, Kehl, Bose et al
Rapid, semiautomated, and fully automated multiplex real-time RT-PCR assays were developed and validated for the detection of influenza (Flu) A, Flu B, and respiratory syncytial virus (RSV) from nasopharyngeal specimens. The assays can detect human H1N1, H3N2, and swine-origin (S-OIV) H1N1 Flu A...
Rx: "PCR for Everything" Encounters Between Clinicians and Novel Diagnostic Tests
07 Dec 2012 | Publications | Contributor(s): Blaschke
Presentation Objectives•Discuss the potential impact/complications of novel diagnostic testing from a clinician point of view•What are the clinical advantages of the new tests?•Are there data to support that the tests are meeting these goals?•What are some of the problems seen and/or anticipated...
New Technology: Is the Juice Worth the Squeeze?
07 Dec 2012 | Publications | Contributor(s): Ginocchio
Program Objectives•Explain the advantages and disadvantages of highly multiplexed testing•List clinical applications where multiplex testing should be beneficial•List the indications and uses for multi-analyte respiratory virus detection•Evaluate your testing needs and economical impact of testing
DISINFECTANTS AND ASTHMA
13 Apr 2013 | Publications | Contributor(s): Kenneth D Rosenman, Mary Jo Reilly
Michigan State University's College of Human Medicine produces a report on the literature review on some key chemical disinfectants and their potential to cause asthma. Sufficient medical documentation shows that individuals can become sensitized and develop asthma from exposure to formaldehyde,...
A ''Genome-to-Lead'' Approach for Insecticide Discovery: Pharmacological Characterization and Screening of Aedes aegypti D1-like Dopamine Receptors
18 Apr 2013 | Publications | Contributor(s): Jason M. Meyer, Karin F. K. Ejendal, Larisa V. Avramova, Elisabeth E. Garland-Kuntz, Gloria I. Giraldo-Caldero´n, Tarsis F. Brust, Val Watts, Catherine A. Hill
Background: Many neglected tropical infectious diseases affecting humans are transmitted by arthropods such asmosquitoes and ticks. New mode-of-action chemistries are urgently sought to enhance vector management practices incountries where arthropod-borne diseases are endemic, especially where...
Starvation causes disturbance in amino acid and fatty acid metabolism in Diporeia
18 Apr 2013 | Publications | Contributor(s): Suman Maity, Amber Jannasch, Jiri Adamec, Thomas Nalepa, Tomas O. Höök, Maria S. Sepúlveda
The benthic amphipod Diporeia spp. was once the predominant macroinvertebrate in deep, offshore regions of the Laurentian Great Lakes. However, since the early 1990s, Diporeia populations have steadily declined across the area. It has been hypothesized that this decline is due to starvation from...
Crystal structure and characterization of coiled-coil domain of the transient receptor potential channel PKD2L1
18 Apr 2013 | Publications | Contributor(s): Katrina L. Molland, Lake N. Paul, Dinesh A. Yernool
The cation-permeable channel PKD2L1 forms a homomeric assembly as well as heteromeric associations with both PKD1 and PKD1L3, with the cytoplasmic regulatory domain (CRD) of PKD2L1 often playing a role in assembly and/or function. Our previous work indicated that the isolated PKD2L1 CRD...
Development and optimization of an LC-MS/MS-based method for simultaneous quantification of vitamin D2, vitamin D3, 25- hydroxyvitamin D2 and 25-hydroxyvitamin D3
18 Apr 2013 | Publications | Contributor(s): Jiri Adamec, Amber Jannasch, Jianjie Huang, Emily Hohman, James C. Fleet, Munro Peacock, Mario Ferruzzi, Berdine Martin, Connie Marie Weaver
Simultaneous and accurate measurement of vitamin D and 25-hydroxyvitamin D in biological samples is a barrier limiting our ability to define “optimal” vitamin D status. Thus, our goal was to optimize conditions and evaluate an LC-MS method for simultaneous detection and quantification of vitamin...
Development of a new method for improved identification and relative quantification of unknown metabolites in complex samples: Determination of a triterpenoid metabolic fingerprint for the in situ characterization of Ganoderma bioactive compounds
18 Apr 2013 | Publications | Contributor(s): Jiri Adamec, Amber Jannasch, Shailesh Dudhgaonkar, Andrej Jedinak, Miroslav Sedlak, Daniel Sliva
Ganoderma lucidum is a mushroom with a long history of medical applications. Research has demonstrated chemotherapeutic effects of G. lucidum in tissue culture, and bioactive fractions of the mushroom have been shown to contain high levels of triterpenoids and polysaccharides. In this study, we...
Folding dynamics of phenylalanine hydroxylase depends on the enzyme's metallation state: the native metal, iron, protects against aggregate intermediates
18 Apr 2013 | Publications | Contributor(s): Aristobulo Loaiza, Judith A Ronau, Alexander Ribbe, Lia Stanciu, John Burgner II, Lake N. Paul, Mahdi M. Abu-Omar
Phenylalanine hydroxylase (PAH), a non-heme iron enzyme, is responsible for the phenylalanine conversion to tyrosine. Its malfunction causes phenylketonuria (PKU). To better understand how protein structure and folding profiles are affected by the metal cofactor, we investigated the chemical...